zoe fluorescent cell imager  (Bio-Rad)

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: Anticoagulant performance of surface coatings. (A) Protein adsorption on the surfaces. (B) Platelet adhesion density quantified from (C) fluorescence images of DiOC6-stained platelets. Clotting times determined using (D) APTT and (E) TT assays. Statistical comparisons of samples with PET were performed using a one-way ANOVA test.

Article Snippet: Fluorescence images were acquired using an inverted microscope (CKX53, Olympus Corporation, Japan) and cell viability was quantified based on the FI of calein AM measured at an excitation wavelength of 496 nm and an emission wavelength of 516 nm. (1) Cell viability ( % ) = FI sample − FI blank FI control − FI blank × 100 %

Techniques: Adsorption, Fluorescence, Staining, Coagulation

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro antithrombosis performance under flow conditions. (A) Merged images visualising the anticoagulant capability of the coated surfaces compared to uncoated surfaces and (B) DiOC6 fluorescence intensity measured over time. Bright-field images were merged with Cy5-labelled coatings (red) and DiOC6-stained platelets (green) to enable simultaneous visualisation of the surface layer and platelet adhesion. Statistical analyses were performed between data points at the same time interval for the two groups using a t -test. (C) Schematic representation of the thrombolysis assay conducted on a microfluidic device. (D) Images showing thrombus formation at the inlet of the microfluidic device under flow for 5 h, captured before and after laser irradiation for thrombus degradation. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Fluorescence images were acquired using an inverted microscope (CKX53, Olympus Corporation, Japan) and cell viability was quantified based on the FI of calein AM measured at an excitation wavelength of 496 nm and an emission wavelength of 516 nm. (1) Cell viability ( % ) = FI sample − FI blank FI control − FI blank × 100 %

Techniques: In Vitro, Fluorescence, Staining, Irradiation